MU-MMRRC - Genotyping

The gut microbiota (GM) is now recognized as a key factor in health and disease. Differences in the composition of the gut microbiota of research animals may contribute to altered phenotypes or poor reproducibility of animal-based studies. Culture-independent techniques such as next-generation sequencing (NGS) allow in-depth characterization of the gut microbiota. The MMRRC now offers a full line of NGS-related services including fecal DNA extraction, 16S rDNA gene sequencing, and informatics analysis to researchers using mouse models. For more information, please see here or contact mmrrc@missouri.edu.

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MU METAGENOMICS CENTER (MUMC)

Journal of the Missouri State Medical Association

June 01, 2013

SCIENCE OF MEDICINE
One Medicine: Collaborative Research on Human & Animal Disease for the Betterment of Both
Craig L. Franklin, DVM, PhD .......... 195

A Brief History of Animal Modeling
Aaron C. Ericsson, DVM, PhD, Marcus J. Crim, DVM & Craig L. Franklin, DVM, PhD .......... 201

New York Times Article Misleads on the Value of Mouse Models
Catherine E. Hagan, DVM, PhD .......... 206

The Mighty Mouse: The Impact of Rodents on Advances in Biomedical Research
Elizabeth C. Bryda, PhD .......... 207

The Laboratory Animal Veterinarian: More than just a Mouse Doctor
Cynthia G. Alvarado, DVM & Lonny M. Dixon, DVM .......... 223

January ,

Genotyping

Genotyping requires tail tip, ear punch or sperm aliquot. Types of assays available include standard PCR, standard PCR followed by restriction digest or sequencing, RT-PCR, qPCR and probe-based allelic discrimination.

Genotyping assay development requires certain sequence information and a positive control sample. Please contact us to discuss.

Validation/optimization of a genotyping assay requires an assay protocol with primer information and a positive control sample.

Microsatellite Analysis: Low density microsatellite or SNP analysis is available for genotyping, strain identification or marker-assisted speed congenic approaches.

SERVICES OFFERED	Non-Profit/ Academic Fees	For-Profit Fees
Genotyping (per sample)	$20	$30
Genotyping assay development	$250	$500
Genotyping assay validation	$119	$175
Microsatellite analysis (for speed congenics)	Contact for Quote

Reproducibility White Paper

The MU Metagenomics Center recently launched a new website – please visit us at:

http://mumc.missouri.edu Watch the video!

http://mumc.missouri.edu
Watch the video!