for assistance call: 1-888-673-3444
 
MMRRC & REPRODUCIBILITY!
 June 16, 2015
NATURE news:  

Reproducibility White Paper

New MUMC Website!
 April 06, 2015

The MU Metagenomics Center recently launched a new website – please visit us at:

http://mumc.missouri.edu
Watch the video!

Microbiota Characterization
 April 02, 2015
The gut microbiota (GM) is now recognized as a key factor in health and disease.  Differences in the composition of the gut microbiota of research animals may contribute to altered phenotypes or poor reproducibility of animal-based studies.  Culture-independent techniques such as next-generation sequencing (NGS) allow in-depth characterization of the gut microbiota.  The MMRRC now offers a full line of NGS-related services including fecal DNA extraction, 16S rDNA gene sequencing, and informatics analysis to researchers using mouse models.  For more information, please see here or contact mmrrc@missouri.edu.

Please visit
MU METAGENOMICS CENTER (MUMC)

Journal of the Missouri State Medical Association
 June 01, 2013

SCIENCE OF MEDICINE
One Medicine: Collaborative Research on Human & Animal Disease for the Betterment of Both
Craig L. Franklin, DVM, PhD .......... 195

A Brief History of Animal Modeling
Aaron C. Ericsson, DVM, PhD, Marcus J. Crim, DVM & Craig L. Franklin, DVM, PhD .......... 201

New York Times Article Misleads on the Value of Mouse Models
Catherine E. Hagan, DVM, PhD .......... 206


The Mighty Mouse: The Impact of Rodents on Advances in Biomedical Research
Elizabeth C. Bryda, PhD .......... 207

The Laboratory Animal Veterinarian: More than just a Mouse Doctor
Cynthia G. Alvarado, DVM & Lonny M. Dixon, DVM .......... 223

Services

In addition to providing mutant mouse strains submitted to the MMRRC, the MU center also can provide the following to assist with your mutant mouse needs:

SERVICES OFFERED Non-Profit/ Academic Fees For-Profit Fees

Cryopreservation

 

 

Sperm cryopreservation

$425

$625

Embryo cryopreservation

$945

$1,390

Cryo-storage

 

 

Sperm /embryo storage (strain per year)

$306

$438

Cryo-Resuscitation

 

 

Resuscitation via  cryopreserved embryos

$3,428

$5,040

Resuscitation via cryopreserved sperm with good post-thaw motility

$4,462

$6,562

Resuscitation via cryopreserved sperm with poor post-thaw motility

$9,308

$13,688

Rederivation

 

 

Rederivation to pathogen-free status via embryo transfer

Contact for Quote

Genotyping

 

 

Genotyping (per sample)

$20

$30

Genotyping assay development

$250

$500

Genotyping assay validation

$119

$175

Microsatellite analysis (for speed congenics)   

Contact for Quote

Live mice and tissues

 

 

Timed pregnant females

$1,478

$2,157

Tissue/Organ sample collection

$459

$670

Colony Management

Contact for Quote

Microbiota Characterization

 

16S rDNA gene sequencing

Contact for Quote

 


Cryopreservation


Sperm cryopreservation requires submission of 3-4 males, preferably proven breeders, 10-26 weeks of age is ideal. You will receive frozen sperm aliquots in sterile CBS high-biosecurity straws.
Embryo cryopreservation requires submission of 8-10 males, preferably proven breeders, 10-26 weeks of age, and submission of 24-32 females, preferably 7-14 weeks of age. You will receive all cryopreserved morula-stage embryos collected from the donor females you provide (~20 embryos per sterile CBS high-biosecurity straws).



Cryo-Resuscitation


Resuscitation via cryopreserved embryos requires submission of 40-60 cryopreserved embryos.  You will receive all recovered pups after genotyping and health monitoring procedures are performed.
Resuscitation via cryopreserved sperm that has good post-thaw progressive motility and concentration will be performed by in-vitro fertilization (IVF).  You will receive all recovered pups after genotyping and health monitoring procedures are performed.
Resuscitation via cryopreserved sperm that has poor post-thaw motility and/or low sperm concentration will be performed by intracytoplasmic sperm injection (ICSI).  You will receive all recovered pups after genotyping and health monitoring procedures are performed.



Rederivation


Rederivation to pathogen-free status via embryo transfer requires submission of 5-6 males, ideally 10-26 weeks of age and 10-12 females, ideally 7-14 weeks of age to use as oocyte-donors. You will receive all recovered pups after genotyping and health monitoring assays are performed.

Resuscitation and rederivation procedures are performed in pathogen-free barrier facility, and clean surrogate mothers purchased from commercial vendor are used for embryo transfers. You will receive the litters with confirmed genetics and pathogen-free health status.



Genotyping


Genotyping requires tail tip, ear punch or sperm aliquot.  Types of assays available include standard PCR, standard PCR followed by restriction digest or sequencing, RT-PCR, qPCR and probe-based allelic discrimination.
Genotyping assay development requires certain sequence information and a positive control sample.  Please contact us to discuss.
Validation/optimization of a genotyping assay requires an assay protocol with primer information and a positive control sample.
Microsatellite Analysis:  Low density microsatellite or SNP analysis is available for genotyping, strain identification or marker-assisted speed congenic approaches.



Live mice and tissues


Timed pregnant females are available at this price for our live colony strains only.
Tissue or organ sample collection from specific strains, preserved or fixed to your specifications. Cost includes euthanasia, necropsy and aseptic collection of one organ or tissue.  Additional samples may be collected from same animal for $10 per organ or tissue.
Colony Management accepting a breeding nucleus of mice and maintaining a colony that will produce mice for research, will include per diem and labor fees. This may also include fees for genotyping of offspring as well as a variety of other concerns, such as specialty diet, husbandry, and/or veterinary attention. Please contact us to discuss.



Microbiota Characterization


16S rDNA gene sequencing requires submission of 2 fresh fecal samples per mouse.  Samples should be frozen immediately following collection and shipped frozen.  You will receive a detailed report containing the relative abundance of all detected microbes at each taxonomic level, 2D and 3D principal component analyses, rarefaction curves, and the associated raw data.  For more information, please see here.



Important notes


All MU-MMRRC services are performed with the highest scientific and biosecurity standards, and the above listed fees defray the costs associated with these services.

The procedures performed with the use of cryopreserved materials are not 100% guaranteed.  However, MU-MMRRC has very high success rates (95-100%) if sperm or embryos were cryopreserved by us or optimally cryopreserved by the other institutions.

Fees include genotyping assays and health monitoring of surrogate mothers.  See Colony Management for health monitoring procedures. Additional cryopreservation, resuscitation or rederivation attempts are subject to extra charges and fees for these services will be determined on a case by case basis. A processing fee of $200 will be added to all international orders.  All fees are in U.S. dollars and do not include any shipping charges.